Coding

Part:BBa_K5090001:Design

Designed by: Manuel Marques Vilar   Group: iGEM24_Stony-Brook   (2024-09-30)


Enhanced LacI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1081
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1081
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1081
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1081
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1081
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We implemented this part based on the sequence by Wang et al. (2023). We made no change, including not removing SV40 NLS so as to keep our implementation as similar to the original as possible even given the new bacterial context.


Source

https://www.thno.org/v13/p2552/thnov13p2552s1.pdf

References